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Leucine dehydrogenase
Reference: LED-201

Leucine dehydrogenase

Leucine dehydrogenase from Bacillus sp. L-Leucine + H2O + NAD+ ----> α-Ketoisocaproate + NH3 + NADH + H+ L-Leucine + H2O + NAD+ <---- α-Ketoisocaproate + NH3 + NADH + H+ This enzyme is used for enzyme determination of L-leucine and the activity of leucine aminopeptidase.
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DESCRIPTION
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  • Product name: L-Leucine: NAD+ oxidoreductase (deaminating)
  • Appearance: White amorphous powder lyophilized
  • Activity: Grade Ⅱ, 20U/mg-solid or more (containing approx. 70% of stabilizers)
  • Contaminants: Leucylpeptide decomposing enzymes :
    (Leu-Val) ≤ 1.0×10-2%
    (Leu-Gly-Gly) ≤ 1.0×10-2%
    NADH oxidase ≤ 1.0×10-2%
  • Stabilizers: 2-Mercaptoethanol, L-cysteine, dithiothreitol, ethylenediaminetetraacetate
  • Stability: Stable at －20℃ for at least one year
  • Molecular weight: 245,000
  • Michaelis constants: 1.0×10-3M (L-Leucine), 3.9×10-4M (NAD＋), 3.5×10-5M, (NADH), 3.1×10-4M［α-Ketoisocaproate (α-K Ⅰ C)］, 2.0×10-1M (NH3)
  • Structure: 6 subunits per mol of enzyme
  • Inhibitors: Na2S, Hg2+, Cu2+, Co2+, Mg2+, p-chloromercuribenzoate
  • Optimum pH: 10.5－10.8 (L-Leu→α-KⅠC), 9.4 (α-K Ⅰ C→L-Leu )
  • Optimum temperature: Above 70℃
  • pH Stability: pH 5.5－10.5 (25℃, 20hr)
  • Thermal stability: Below 60℃ (pH 6.9, 10min)
  • EC # 1.4.1.9
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