Sorachim
Reference: LED-201

Leucine dehydrogenase

Leucine dehydrogenase from Bacillus sp. L-Leucine + H2O + NAD+ ----> α-Ketoisocaproate + NH3 + NADH + H+ L-Leucine + H2O + NAD+ <---- α-Ketoisocaproate + NH3 + NADH + H+ This enzyme is used for enzyme determination of L-leucine and the activity of leucine aminopeptidase.
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DESCRIPTION
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  • Product name L-Leucine: NAD+ oxidoreductase (deaminating)
  • Appearance White amorphous powder lyophilized
  • Activity GradeⅡ 20U/mg-solid or more (containing approx. 70% of
    stabilizers)
  • Contaminants Leucylpeptide decomposing enzymes :
  • (Leu-Val) ≤ 1.0×10-2%
  • (Leu-Gly-Gly) ≤ 1.0×10-2%
  • NADH oxidase ≤ 1.0×10-2%
  • Stabilizers 2-Mercaptoethanol, L-cysteine, dithiothreitol,
  • ethylenediaminetetraacetate
  • Stability Stable at -20℃ for at least one year
  • Molecular weight 245,000
  • Michaelis constants 1.0×10-3M (L-Leucine), 3.9×10-4M (NAD+), 3.5×10-5M
  • (NADH), 3.1×10-4M[α-Ketoisocaproate (α-KⅠC)],
  • 2.0×10-1M (NH3)
  • Structure 6 subunits per mol of enzyme
  • Inhibitors Na2S, Hg2+, Cu2+, Co2+, Mg2+, p-chloromercuribenzoate
  • Optimum pH 10.5-10.8 (L-Leu→α-KⅠC), 9.4 (α-KⅠC→L-Leu )
  • Optimum temperature Above 70℃
  • pH Stability pH 5.5-10.5 (25℃, 20hr)
  • Thermal stability Below 60℃ (pH 6.9, 10min)
  • EC # 1.4.1.9

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